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Meaning:
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a dirty mark or patch of colour; a moral defect; (to cause something) to change colour, make coloured patches or dirty marks on (something)

spot of dirt, blot, bar

blemish blotch color dye smudge speck splotch stigma tint blur brand discoloration disgrace dishonor drip infamy mottle odium onus reproach shame slur smirch spatter spot black eye ink spot sinister
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Cases of word usage

1) a mark, stain etc that spoils something (blot)

2) a line, scratch, cut, stain etc that spoils the appearance of something; a sign or indication (mark)

STAIN as in Wiktionary
STAIN as in Wikipedia